Description |
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has a high quantum efficiency and a large Stokes shift. It is well excited at 488 nm with its maximum emission peak at 677nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting. Its cyanine tandem conjugates (such as PerCP-Cy5.5) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis. |
Form |
Lyophilized |
Molecular Weight |
35 kDa |
Absorption Maximum |
477 nm |
Emission Maximum |
678 nm |
Extinction Coefficient |
4.06×10^5cm -1 M-1 |
Reconstitute |
Reconstitute whole bottle of SMCC-PerCP with your conjugate buffer to adjust the concentration for further use. |
Buffer |
Lyophilized SMCC-PerCP powder is prepared in 10 mM K-P buffer with sugar as additive. No ammonium sulfate or other material that may interfere conjugation is contained in this product. |
Spectra |
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