Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit
总抗氧化能力(T-AOC)比色法测试盒
| 规格： | / 50 assays(25 samples) / 100 assays(50 samples) |
| 价格： | / ￥320 / ￥580 |
货号：E-BC-K136-S
检测仪器及波长: 紫外-可见光分光光度计（520 nm）
样本类型: 血清（浆）;唾液;尿液;组织;细胞
检测原理
机体中有许多抗氧化物质，能使三价铁离子还原成二价铁离子，后者可与菲啉类物质形成稳固的橙红色络合物，通过比色可测出其抗氧化能力高低。
关键信息
| 指标简称 | T-AOC |
| 样本类型 | 血清（浆）;唾液;尿液;组织;细胞 |
| 灵敏度 | 0.62 U/mL |
| 检测范围 | 0.62-145.2 U/mL |
| 精密度 | 批间差：8.2%批内差：2.7% |
| 检测仪器及波长 | 分光光度计 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个差异大的样本稀释成不同浓度进行预实验，结合本试剂盒的检测范围（0.62-145.2 U/mL），选取最佳稀释倍数，进行正式批量实验。不同样本的稀释比例范围如下表（仅供参考）:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
10%小鼠肝匀浆 |
不稀释 |
猪血清 |
不稀释 |
|
10%小鼠肺匀浆 |
不稀释 |
大鼠血浆 |
不稀释 |
|
人唾液 |
不稀释 |
HepG2细胞 |
不稀释 |
|
人血清 |
不稀释 |
|
|
注：稀释液为生理盐水（0.9% NaCl）或PBS（0.01 M，pH 7.4）。
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