VEGFA Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-53277
宿主: Rabbit
反应性: H
应用: WB,IHC
Overview
| Synonyms | Folliculostellate cell-derived growth factor,Glioma-derived endothelial cell mitogen,MGC70609,MVCD1,Vascular endothelial growth factor A,vascular endothelial growth factor A121,vascular endothelial growth factor A165,vascular endothelial growth factor,Vascular permeability factor,VEGF A,Vegf,VEGF-A,VEGF120,Vegfa,VEGFA,VPF,VEGFA抗体 |
| Swissprot | P15692 |
| Source | Rabbit |
| Reactivity | Human |
| Immunogen | Synthetic peptide of human VEGFA |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:1000-1:5000;IHC,,1:30-1:150; |
| Concentration | 1.8mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Background | This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. |
Images
Western blot analysis of 293T cell lysate using VEGFA Polyclonal Antibody at dilution of 1:1450
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using VEGFA Polyclonal Antibody at dilution of 1:60(×200)
Immunohistochemistry of paraffin-embedded Human brain tissue using VEGFA Polyclonal Antibody at dilution of 1:60(×200)
